Posts

I found myself to force to use cellranger. Meanwhile it helps a lot to run from bcl files to single cell counts matrixes, I discovered …
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We use bcbio-nextgen for the analysis of sequencing data, mainly, (sc)RNAseq, smallRNAseq, DNASeq and ChIPSeq. It is not rare that we …
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This post will show how to configure quickly the colors for the annotation of rows/columns that go on top or on the side of a heatmap. …
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This post should show you an easy way to get the following data type integrated into a figure: functional enrichment analysis gene …
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This is a funny story, and I will try to tell you how I realized I don’t know anything about R in 400 words. I work at the …
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Differentially gene expression analysis with RNA-seq data is quite common nowadays, and there are pretty good Bioconductor packages for …
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In summary: I will show which is the best miRNA mapping tool. I used several options for this benchmarking: miraligner from SeqBuster …
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My PhD was focused on small RNA sequencing data. I had a problem when I wanted to visualized the amount of small RNAs from the …
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What is the lying factor in figures? It is the ratio between the difference of two numbers and the difference of the visualization of …
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Everybody who is working with microRNA knows about miRBase, it was the first miRNA catalogue. Everybody is using it to annotate small …
4 min read