Latest posts covering bioinformatics methods, computational biology insights, and technology updates.
Last Friday, I had the chance to join a panel discussion on advancing diversity in challenging times. I was lucky to be joined by two brilliant next-gen scientists, Francince Camacho and Saba Nafees. Follow them—they’re going to be incredible leaders and influencers in our field and beyond.
I found myself to force to use cellranger. Meanwhile it helps a lot to run from bcl files to single cell counts matrixes, I discovered that is quite difficult to control many options related to optimization.
We use bcbio-nextgen for the analysis of sequencing data, mainly, (sc)RNAseq, smallRNAseq, DNASeq and ChIPSeq. It is not rare that we get collaborators who wants to re-analyze public data-set.
Inside bcbio, we have bcbio_prepare_samples.py
to help to merge multiple
files that belong to the same sample into one file to make easier the configuration
of bcbio. We extended this script to pull down data from GEO and
SRA repository.